Methylated active form of vitamin B12
The methylated, biologically active form of vitamin B12 used as a research reference standard in metabolic, neurological and haematology pharmacology.
| Compound | Methylcobalamin (B12) |
| Research category | Cofactor Research |
| Available sizes | 10mg |
| Pricing | From £15 |
| Format | Lyophilised powder in sealed vial |
| Purity | >99% by HPLC (Janoshik Analytical, batch-verified) |
| Intended use | In-vitro laboratory research only. Not for human consumption. |
Reference data drawn from public databases (PubChem, UniProt) and peer-reviewed literature. For research and laboratory characterisation only.
| Compound class | Methylcobalamin - methylated form of vitamin B12 |
| Molecular formula | C63H91CoN13O14P |
| Molecular weight | 1344.4 g/mol |
| CAS Registry Number | 13422-55-4 |
| PubChem CID | 6436231 |
| Receptor / mechanism | Methyl-donor cofactor for methionine synthase. |
| Reported half-life | Reported ~6 days in human serum. |
| Solubility | Lyophilised powder reconstitutes in bacteriostatic water for laboratory use. |
| Storage (lyophilised) | Stable at 2–8 °C lyophilised. Light-sensitive - store in amber vial. |
| Storage (reconstituted) | Reconstituted: store at 2–8 °C, use within 30 days. Protect from light. |
| Clinical / regulatory status | Endogenous cofactor; widely used in research. |
Methylcobalamin is a bioactive form of vitamin B12 characterised in the biochemical literature as a cofactor in methionine synthase. Supplied as lyophilised powder for in-vitro research use only.
The above describes how this compound is characterised in the published peer-reviewed literature. It is not a claim about effects in humans.
Methylcobalamin (B12) is supplied as a lyophilised (freeze-dried) powder in a sealed glass vial. For laboratory reconstitution, add the desired volume of diluent (typically bacteriostatic water) slowly down the side wall of the vial. Swirl gently until fully dissolved - do not vortex or shake. Store reconstituted solutions at 2–8 °C and use within 4–6 weeks.
Use our Reconstitution Volume Calculator to compute the resulting mg/ml concentration.
| State | Temperature | Shelf life |
|---|---|---|
| Lyophilised | 2–8 °C or −20 °C | Up to 24 months |
| Reconstituted | 2–8 °C | 4–6 weeks typical |
Every batch of Methylcobalamin (B12) we supply is independently HPLC-verified by Janoshik Analytical. Where a Janoshik report has been issued for the current batch it is supplied with the order and carries a unique verification key that can be authenticated against Janoshik's records. Reported purity is consistently above 99%.
Researchers working with Methylcobalamin (B12) often work alongside the following compounds in the preclinical literature: